Real-Time PCR (qPCR)
Real-Time Polymerase Chain Reaction (qPCR) is a molecular biology technique used to amplify and simultaneously quantify DNA or RNA targets. A qPCR thermocycler controls temperature cycles for denaturation, annealing, and extension while monitoring fluorescence signals in real time. It provides rapid, sensitive, and accurate detection of nucleic acids. qPCR is widely used in gene expression analysis, pathogen detection, genetic testing, clinical diagnostics, and research applications due to its high specificity and quantitative capabilities.
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